The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Molar Ratio: ~5.5
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogen
Purified S-Acetyl Coenzyme A Synthetase from baker's Yeast
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Target
ACSS2
(Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))
Alternative Name
ACSS2
Background
Acetyl Coenzyme A Synthetase is a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. It acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: ACAS2, ACSA, Acetate-CoA ligase, Acetyl-CoA synthetase, Acetyl-coenzyme A synthetase cytoplasmic, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme